Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microplate Readers by issuing a single SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. 305 reference level saturation. Turn on Plate Reader and wait for it to complete its start-up routine. Category 2 and 3 microplate readers use gradient heating as well. Cells stably expressing wild-type or E633K p110β were. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. If the computer is connected to the Internet: Select Search Automatically for. Luminescence was recorded in a Molecular Devices SpectraMax M5 plate reader using SoftMax Pro. Extracting viral DNA from 4 x 109 copies of the AAV8 preparation ( nominally 5 ng ssDNA)The SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. Microplate Reader Shelf. 5. Developed with Promega reagents to provide a. The StakMax stacker also allows users to feed plates into the loading area manually. 2. Plate Reader (3) Name Price; EnSpire Flexstation 3 SpectraMax M5 Plate. For example, microplates containing different samp les can be read using the same orChoose the desired plate type, using the upper dropdown menu to choose plate format (96 or 384 wells) and the "Select Specific" menu to choose the specific plate type. Supernatants were collected, and the concentration of l-lactate was determined by measuring absorbance of reaction samples using the Spectra Max M5 plate reader (Molecular Devices). The plate reader. A. 2. 17. 1. Molecular Devices Spectramax M5 Microplate Reader; Related products. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. 5 pM (0. Absorbance: For wavelengths in the visible range, 340 nm and above, clear polystyrene plates are recommended. The 2018 M5 is powered by a 600-hp twin-turbocharged 4. Products : SpectraTest Validation Plates ABS2, SpectraTest Validation Plates ABS1, SpectraTest Validation Plates FL1, SpectraTest Validation Plates LM1. 5 and 10 nm were used for excitation and emission, respectively. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. Gemini XPS Reader optics. Cytation 5 combines automated microscopy and conventional microplate detection in a configurable, upgradable platform. The plates were centrifuged at the end of incubation, and fluorescent signals in supernatants were measured using a SpectraMax™ M5 microplate reader, with excitation at 485 nm and emission at 520 nm. Standards and QCs are composed of a. Qualitative and quantitative data analysis, along with customizable exporting and reporting enable automated end-to. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. 0 adjusted at rt containing 10% v/v DMSO) (100 μL) and a 2- or 5-fold dilution was. 5. 2 mg/mL using 2 different UV-visible spectrophotometers, a SpectraMax M5 plate reader, and a NanoDrop instrument. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 1. The plate reader can measure multiple readouts, including UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Lastly, Jurkat cells were least susceptible to Vorinostat treatment (all IC 50 > 50. edu, 814-863-3503. Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates. Absorbance measured on a Spectra Max M5 plate reader (Molecular Devices). Then the plate was incubated at 37 °C for 2 h. Download a PDF version of the SpectraTest validation package. 5°C at 37°C. Page 8 SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide CAUTION! Use of organic solvents can cause harm to the optics in the instrument. Microplate Readers; SoftMax Pro Software; Microplate Stacker and Washer; Cellular Imaging Systems; Clone Screening; FLIPR; AXON Patch-Clamp; Assay Kits. plates can be used in the SPECTRAmax PLUS 384. For wavelengths in the UV range above 220 nm, UV-clear plastic plates or quartz plates should be used. 25 For direct lysis of clots by plasmin, plasmin (250nM) was added to the surface of clots and lysis monitored by theSteady-state affinity measurements were performed on an Analyst AD, Analyst HT, or SpectraMax M5 plate reader (Molecular Devices). 19 , 25 Human umbilical cord endothelial cells (ATCC, Manassas, VA) were grown in 24-well plates coated with. For. 5. 5 × 10 5 counts/fmol when measured with a Spectromax M5 plate reader using white opaque 96-well plates. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microplate Readers by issuing a single NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Plate section without PathCheck applied. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. 3) At the bottom of the strip, click on "Show. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. This is a result of the light being attenuated and scattered by the. 3) At the bottom of the strip, click on "Show. doi: 10. Chemistry Department Facilities. and 72 h using the CCK-8 protocol with absorbance readings at 450 nm measured with a SpectraMax M5 plate-reader (Molecular Devices. A reaction volume of 12 µL was placed into each well of 384-well clear bottom black-walled plate that was sealed with an oxygen impermeable membrane. 0. Primary human airway. BioTek Microplate Reader. The medium fluid was, respectively, collected at 4. NanoLuc substrate and R3/I5-SmBiT were added to the homogenate of HEK293T cells transiently overexpressing sLgBiT-fused receptor and bioluminescence was measured on a SpectraMax M5 plate reader with an interval of 30 s. Wells with an OD 600[0. Find regional support. edu The content of this site is published by the site owner(s) and is not a statement of advice, opinion, or information pertaining to The Ohio State University. SpectraTest ABS2 Absorbance Validation Package performance tests. The supernatants collected in Subheading 3. View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. 0200-7027. a validation plate, certificate of calibration, user guide, and automated SoftMax® Pro Software protocols to run validation tests. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. 3. IMAP® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. HTRF-compatible SpectraMax Multi-Mode Readers. 5. Subsequently, 200 μL of filtrate was transferred into a 96-well UV Star microplate, and the protein concentration in each filtrate was determined using a SpectraMax M5 UV-Visible plate reader. The samples were loaded into a rectangular quartz cell with the light path length of 1 mm. To detect the luminescent signals, 100 μl of each dilution was added into a 96-well plate before adding 50 μl of LumiGLO Peroxidase Chemiluminescent substrate (KPL, 54-71-00) to measure luminescent intensity by a SPECTRAmax M5 plate reader. 5. 33 Hz acquisition rate (one acquisition every 2 sec plus 1 sec for mixing in between each acquisition), using 495 and 585 nm. Table of Contents. com 10. All values reported represent the mean of at least three independent experiments. 9. Luminescence was measured on a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA) after the addition of nano-Glo substrate (Promega, Madison, WI) in accordance with the manufacturer’s directions. 0 and room temperature. Published Dec 16, 2020 | Updated Jun 22, 2023. This unit must survive. Linear, Orbital Slow, and Orbital Fast. Protein concentration of viral suspensions was measured using a Pierce BCA Protein Assay Kit (23225, Thermo Fisher Scientific) with a mucin standard curve read on a SpectraMax M5 plate reader (Molecular Devices). Accordingly there are different configurations possible. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). For dissociation measurement, competitor R3/I5 was added and bioluminescence was continuously. Flow cytometry analysis H22 cells (5 × 105 cells/well) were seeded in 6-well plates and then cells were treated with or without TB-TF (5, 10, 20, 40, 80 μg/mL) for 48 h. Then, absorbance was measured by a Spectra Max ™ M5 plate reader (Molecular Devices, Sunnyvale, CA). 6 for further experiment, so I'm confused which i would choose for OD value determination. Protocol for Qubit DNA HS Assay. Uncontrolled Copy for Training and Reference Purposes Only . 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. After the plate is read, data will appear in the plate section: 10. Fluorescence was measured using microplate reader at an excitation wavelength of 488 nm and emission wavelength of 529 nm using Spectramax-M5 plate reader (Molecular Devices, Sunnyvale, CA, USA). The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. Each microtiter plate was sampled three times on different days, and each of the recipient plates were separately assayed with a BioTek Synergy2 microplate reader. Download : Download full-size image; Fig. 1. OD 600 and luminescence were measured at 30 minute intervals for 48 hours. 5. 01 M HCl was added, and absorbance was read at 570 nm using a Spectramax M5 plate reader (Molecular Devices). A solution for low volume applications. Description Figure 1. Microtiter plates (Corning 3690; 96-well half area plates) were coated overnight at. 10569The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional. The ultrapure water used in the experiments was prepared with a Millipore Direct-Q® 3 system (Merck Millipore, MA, U. 1. siRNA (750 pmol) was transfected into NRK-49F (1 × 10 6 cells) using a Nucleofector Kit V and the Amaxa Nucleofector. #3635 or Greiner cat. Figure 9. A solution for low volume applications. edu, 814-863-3503. Plate chamber design of the SpectraMax category 1 microplate readers. SpectraMax M5 Plate Reader (Molecular Devices) If you have trouble accessing this page and need to request an alternate format, contact [email protected] H1 Hybrid Multi-Mode Reader Synergy™ H1 is a configurable multi-mode microplate reader. SpectraMax Injector Cartridge with SmartInject™ Technology. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. Thesupplyofhumidifiedair (zero air) was controlled by a solenoid valve with on and offLastly, Jurkat cells were least susceptible to Vorinostat treatment (all IC 50 > 50. 0 out of 5 $ 3,799. 25% (v/v) AlamarBlue reagent was then added to cells and incubated in normal cell culture conditions for 1 h, protected from light. plates in 150 ll OS minimal medium (Schnider-Keel et al. Perkin Elmer Victor X4 Multimode Plate Microplate Reader 2030-0040 with PC. Specifications General -Detection mode Fluorescence, Luminescence -Read method End point, kinetic. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Standard curve for Quant-iT OliGreen ssDNA M5 Plate Reader Assay with 200 µl sample volume Quantification of the AAV 8-GFP-CMV viral DNA using the Quant-iT™ OliGreen® ssDNA reagent (Cat. 0112-0044C 3 Contents Chapter1:CuvettePortValidationOverview 4 CertificateofCalibration 5 ISO-17025 5 AvailableTests 6 Chapter2:UseValidationProtocols 7A SpectraMax M5 plate reader was able to continuously incubate the microtiter plate at 37°C and measure the fluorescence (λex/λex= 529/600 nm) every 5 minutes for 1. Cell Analysis. Activation of reporter constructs was monitored using the Dual-Glo Luciferase assay (Promega) and measured in a Spectramax M5 plate reader. Plate Shaking. CHO-K1 cells were transfected with luciferase. 7°C (gradient heating design). Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. Spectramax M5 plate reader, 5 modes, 200-100 nm Abs range, Abs, FL, Lumi, TRF and FP, Monochromator in 1 nm increments, includes validation and warranty. limit of detection (LLD) for ATP was estimated for each microplate reader using theThe relabeled data may be saved for further analysis as either a . 1) Select the correct plate and the wells that you want to measure. To eliminate the differences of TCR expression levels among different JRT cell lines, relative luminescence unit (RLU) was normalized to the condition with Human T-Activator DynaBeads at 2:1 bead-to-cell ratio. There is currently no charge for using this plate reader. Information on how to reserve the plate readerSunnyvale, California -- October 5, 2017. Sample Format. 2. [4] The IC 50 value of each drug was calculated by the Logit approach. Preparation of biotin–tyramineThe SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. Molecular Devices. 3. EPOCH2 Plate Reader/Spectrophotometer. 1. Menu. 5 pM (0. Thus, the. The plastic from a microplate will affect the light polarization, precluding bottom reads and reading a covered plate. SpectraMax i3x reader measures absorbance, fluorescence, & luminescence with user-upgradeable application including western blot imaging, AlphaScreen, fast kinetics along with injectors plus additional detection modes. The sample is typically placed in a cuvette through which light is sent horizontally. 2. 10 μL of each SCGal-Ura and SC-Ura culture were then transferred to 1 mL fresh SC-URA and allowed to grow to saturation overnight. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes wi. 4 fmol/well, 384-well plate)−bottom Reading Speed (Kinetic) 96 wells: 11 s 384 wells: 22 s Luminescence Wavelength Range 300−700 nm Dynamic Range > 6 decades Sensitivity. reader. : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. 14. StakMax Handling Stacker Base Unit. 3. 01–10 fmol/well (0. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Absorbance was measured at 563 nm using a SpectraMax M5 plate reader (Molecular Devices). Luminescence detection typically offers greater sensitivity than absorbance or fluorescence detection. Animal modelThe PEG precipitation assay was performed with VRC01-WT mAb at a final protein concentration of 0. The colorimetric reaction was stopped with 2 M sulfuric acid, and absorbance was measured at 450 nm using a SpectraMax M5 plate reader (Molecular Devices). Readings were taken every 30 minutes. The guide also covers the software features, the specifications, and the accessories of the systems. between the affordability of filter-based readers and the . This microplate reader handles Absorbance, Fluorescence Intensity, luminescence, Time resolved fluorescence, fluorescence polarization. Catalog number: 165306. The following components are located on the back panel of the SpectraMax and VersaMax readers: • Power switch:The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. 2. The mixed samples were incubated for 5 min at room temperature, and their luminescence intensities were then determined by a SpectraMax M5 plate reader. Temperature control range from Ambient +4º to 50ºC. To monitor the aggregation kinetics, 10 μM ThT was added to a small aliquot (300 μL) of the mixture, and the fluorescence signal (λ ex = 444 nm, λ em = 485 nm) was monitored on a Spectramax M5 plate reader (Molecular Devices). Results were expressed as absorbance units. The TRITC and FITC labeling process is conducted as in a previous study . The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. The SpectraMax ® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. 18632/oncotarget. In the M2, M5 and Plus. Published Dec 16, 2020 | Updated Dec 16, 2020. When a feature or capability applies to only certain readers, this. In general, reading fluorescence from the top is more sensitive than reading from the bottom. Certain functionalities in the table above requires specific configurations. Category 2 and 3 microplate readers use gradient heating as well. Minimize manual manipulation of samples and data processing, and improve laboratory efficiency. The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi-wavelength and. SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 Linearity G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax i3x, SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through. Wolfe1, and Stephen M. 2. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. 1. There is currently no charge for using this plate reader. 2M posts 124. Data were expressed as % control =. Temperature can be set to 4º above ambient to 45°C (thermometer icon) on main toolbar. 1. 5 cm) Weight 80 lbs (36. Page 1 of 8 . : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. One unit of hexokinase is the amount of enzyme that will generate 1. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Answer. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. Thank you!. RUO. The wavelength required to excite the sample can be selected using either a filter of a specific wavelength or a monochromator tuned to the required. Wide range of applications. 320 nm and em = 456 nm) on a Spectramax M5 plate. 3. LbL films are immersed into 20 mM DTT in PBS buffer (2. Bioluminescence assays showed that the engineered Luc-Cys was fully active when compared with 6 × His-NanoLuc prepared in our previous work (Zhang et al. The multi-mode. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400 750 nm for readings in fluorescence polarization mode (M5, M5 e models only). This user guide provides safety information, warnings, cautions, tips, and instructions for operating the instrument, connecting cables, setting up the instrument, and troubleshooting. ABS2, FL1, and LM1 Validation Plates sent to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, an then returned with a new certificate of calibration. 4: Test tube cover. 6-, 12-, 24-, 48-, 96- and 384-well plates. The same procedure. Plate geometry 6- to 384-well plates, Petri dishes Dispense precision ≤2% at 50-200 μL Dispense accuracy ±1 μL or 2% Physical Characteristics Power 250 Watts max. Following color development, absorbance was measured at 450nm using a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA), and BDNF concentrations in samples were calculated using SoftMax Pro software. , Sunnyvale, CA, USA) at the excitation wavelength of 488 nm for carboxy-H2DCFDA and 505 nm for DHE and MitoSOX ™. ameters by UV-Vis assays was done on a multimode SpectraMax M5 plate reader by using 96-well plates (Fisher Scientific). Turn on Plate Reader and wait for it to complete its start-up routine. 9000-0756. Choose the Experiments icon. 40266 Email: [email protected] fluorescence intensity was determined using a fluorescent plate reader (SpectraMax M5 plate reader) set at an excitation wavelength of 485 nm and an emission wavelength of 530 nm. The absorbance was read at 450 nm with 650 nm as background correction using Softmax software on M5 plate reader (Molecular Devices). 2. After the plate is read, data will appear in the plate. Simply unpack it, plug it in, and begin your experiments. During the room temperature incubation (step 9. You can also interpret your results using integrated data analysis software. Reader Documentation Version No. All kinetic reads were taken at 25 °C in nonbinding all black clear-bottomed Greiner 96-well plates covered with optically clear films and stirred for 10 s prior to each reading. You can also interpret your results using integrated data analysis software. The MTS [(3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxy-After this time, cells were stained with crystal violet, washed 3 times and lysed. Add 100 μL of sample media and standards (0, 1, 2, 4, 8, 16, 32, 64, 128 μM NaNO 2) to plate in duplicate. 1. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well. The GloMax® Explorer is a high-performance multimode plate reader that allows you to get up and running quickly, generating the data you need from your experiments. High-performance luminescence, fluorescence, UV-Visible absorbance, BRET and FRET, two-color filtered luminescence and kinetic measurement capabilities. Endothelial permeability was also measured in vitro, using a transwell assay. 1. 312 gain calibration failed. The plate was read by a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA, USA) at 450 nm with 620 nm as reference. Documents. 18632/oncotarget. The BioTek Cytation cell imaging multimode microplate readers offer modularity to meet today’s workflows and are upgradable to meet future workflow requirements. smegmatis using a modified pMV261 vector that allowed protein production upon the addition of 0. Therefore you need to ask a physicist. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Optimizing Fluorescence Polarization Assays Fluorescence polarization for SpectraMax M5 and M5 readers may only be read from the top of a microplate. 2–200 pg/well). Turn the reader on and secure the USB cable. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Assay was run using JAK1 JH1/JH2 and Omnia Y peptide 12 kit. ThT fluorescence was measured at 444 and. FilterMax F3 and F5 Multi. Typical settings for fluorescein are 485/520 and. The GloMax® Explorer is a high-performance multimode plate reader that allows you to get up and running quickly, generating the data you need from your experiments. How-to-use guide for the M5 plate reader and SpectraMax Software in the CMB. 72 MB. Multimode Reader Comparison Chart. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. AB-0558) and immediately read for luminescent signal on a SpectraMax M5 plate reader. Fluorescence values were used to calculate the percent inhibition of enzyme activity relative to maximal activity, PTP4A3 in the absence of inhibitor, and maximal inhibition, PTP4A3 in the presence of 2 mM Na 3 VO 4. 2. For a Price List, please call (858) 646-3100 ext. 3. The optical density at 600 nm (A 600) was monitored using SpectraMax M5 plate reader (Molecular Devices) at specific time intervals. A sensitivity level of 0. Microplate automation and detection instruments for walk away operation and high quality results. The SpectraMax®M3, M4, M5, and M5eMicroplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. 3). This is a result of the light being attenuated and scattered by the. We use Spectramax M5 microplate reader in our lab. After this time period, the plate was read for fluorescence intensity at ex/em wavelengths of 532/590 nm, on a SpectraMax M5 plate reader. After cell attachment, culture media was replaced with F-12 media containing 1% FBS for overnight incubation. Also refer to this Multimode Reader Comparison Chart. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 0. Select Update Driver. Narrow your selection of used microplate readers using the filters above or select the one of the categories on the left to view multi-mode or. 2016 Aug 2; 7(31): 50027–50042. ) and had a resistivity of 18. g. 96-well plate: less than 1 minute; 384-well plate: less than 3 minutes. After 30 min of induction, cells were transferred to a clear-bottom black 96-well plate, and both GFP fluorescence and optical density were measured using a SpectraMax M5 plate reader. Thermo Scientific™ Nunc MicroWell 96-Well Optical-Bottom Plates with Polymer Base have a solid polystyrene black or white upper structures with a polystyrene film at the bottom of the plate. 4: Test tube cover. To the OP, if you are familiar with VAG-COM, you know that RS232-based VAG-COM cables are in the $14 range on eBay. Plates were incubated at 37°C for 6 hours and then equilibrated to room temperature for 30 min. PDE-lux transcriptional fusion plasmids were constructed as previously described. The plate was washed and an anti-rabbit peroxidase-conjugated secondary antibody (Jackson Immunoresearch) was applied at a 1:3000 dilution in PBS. Concentrations of other cytokines were determined by using custom MSD kits and a QuickPlex SQ120 plate reader (Meso Scale Discovery,. Developed with Promega reagents to provide a. Defining the role of the polyasparagine repeat domain of the S. Fuchs1,2,3* 1 Department of Biology, Tufts University, Medford, MA 02155, USA 2 Institute for Protein Innovation, Boston, MA 02115, USA 3 Program in Cellular and Molecular Medicine,. 7°C and the bottom to 36. To update the driver. When the user applies a set temperature of 37°C, the top is heated to 37. Spectranet customer portal GloMax® Discover is a ready-to-use multimode plate reader developed with Promega reagent chemistries to provide a simple means of detecting luminescence, fluorescence and absorbance. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. The plastic from a microplate will affect the light polarization, precluding bottom reads and reading a covered plate. Reactions on 384-well plates (Corning) were monitored by fluorescence (emission 545 nm) using a SpectraMax M5 plate reader (Molecular Devices), with excitation at 274 nm, for 20 min at 2-min. Prepare a 1:1 solution of Greiss Reagent 1:Greiss Reagent 2. SpectraMax M5 Plate Reader (Molecular Devices) If you have trouble accessing this page and need to request an alternate format, contact [email protected]. Place the plate in the SpectraMax M5 plate reader immediately, read OD 405 nm every 30s, last 1 h, 37 °C. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. The SpectraMax® ABS Plus Microplate Reader can run . ZERO BIAS - scores, article reviews, protocol conditions and more. Table of Contents. Viability Assay reagent (Promega). Information on cell morphology, localization of signal, cell count, and more is obtained with Cytation 5 imaging mode. Please talk to your your local Tecan representative for the right configuration which suits your application demands. Windows® 8. FeCl 3, iron standards are always included (0-4 µg /ml). There is currently no charge for using this plate reader. SpectraMax M5 Plate Reader: 901F: Nanodrop 2001: BioRad ChemiDoc gel documentation (old) Bio-Rad T100Tm Thermal Cycler : Bio-Rad CFX ConnectTM Real-Time System : Location. 1 M glycine-phosphate-citrate buffers with 0. Purified fluorescent protein was diluted 15-fold into 0. 3465 or email us. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microp late Readers by issuing a single SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. 303 unable to cal dark current. Description Figure 1. Setup for Z’-LYTE® Assay on SpectraMax® M5/M5e Microplate Reader with SoftMax® Pro 6 Software. Absorbance was measured at 405nm on a Molecular Devices SpectraMax M5 plate reader. Duplicate wells were tested for each condition, and data were graphed as a percent of control in GraphPad Prism (GraphPad Software). Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini XPS reader, SpectraMax M5/M5e reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax M4 reader How does the reader perform a self check?. 4. To avoid these issues and provide the best possible dynamic range between the highest and the lowest measurement values of your assay, fluorescence gain is typically adjusted on the sample with the expected highest signal output (e. After that, the medium was. Detection Modes. Enter Sample IDs (HPV-Type, Sample Description and Data Reference when applicable), Dilution Factors, and background information into the template. That explains the lower values on the LmaxII384 plate reader. a validation plate, certificate of calibration, user guide, and automated SoftMax® Pro Software protocols to run validation tests. Select Start>Control panel>Device Manager. 99 was required for a valid standard curve. Colorimetric detection of crystal violet released into the supernatant, which is proportional to the number of attached cells, was determined using a SpectraMax M5 plate reader (Molecular Devices). Quick Guide to the plate reader. The absorbance at 450 nm was subsequently recorded with SpectraMax M5 plate reader (Molecular Devices). 2. Reader Documentation Version No. We would like to show you a description here but the site won’t allow us. Microplate Reader, Multimode. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. 25 fmol/well, 384-well plate)−top Fluorescein 4 pM (0. Wavelength (s) 200 to 1000 nm (Abs); 250 to 850 nm (Fluor); 250 to 850 nm (TRF) fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400–750 nm for readings in fluorescence polarization mode (M5, M5e models only). Fe standards require only about 30 minutes but can be kept in 1x working solution for at least a week withoutAfter 15 min, the absorbance at 550 nm was recorded using a Molecular Devices SpectraMax M5 plate reader. With optimized reagents, option to operate the readers. 25 pM (0. to meet all your research needs. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. Fast protein liquid chromatography (FPLC) was performed as the last protein purification step on a Bio-Rad BioLogic DuoFlow using a HighPrep TM 26/60 Sephacryl TM S-200 High Resolution column. The SpectraMax 190 reader can be integrated with Molecular . Cytation 5 combines automated microscopy and conventional microplate detection in a configurable, upgradable platform. The lower . 51 L/g). Representative fluorescence images of the cell-containing scaffolds and well plate are shown in Fig. Kojic acid was used as a positive control. Reduce Energy Use by 59% [10] Printing time isn't the only thing getting cut by 70%. a SpectraMax M5 plate reader (Molecular Devices LLC) to detect the absorbance value at 450 nm.